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Description
The HeLa 422 cell line is a subclone of the cell line HeLa. The original HeLa cell line was derived from a cervical carcinoma from a 31 year old female and the HeLa 422 cell line was transfected with ER-pBact, human estrogen receptor cDNA, under control of the human beta actin promotor, resistant to G418S.;
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Description
The HeLa 422 cell line is a subclone of the cell line HeLa. The original HeLa cell line was derived from a cervical carcinoma from a 31 year old female and the HeLa 422 cell line was transfected with ER-pBact, human estrogen receptor cDNA, under control of the human beta actin promotor, resistant to G418S.;
Organism: Homo sapiens, human
Source: cervix
Culture Properties: Monolayer
Morphology: continuous culture, grown as monolayer, morphology epithelial-like
Recommended Medium: EMEM and 10% h.i. FBS
Quality ControlSterility: mycoplasma negative, HOECHST and PCR
Storage and Shipping: Frozen with Culture medium + 50% FBS + 10% DMSO; ship in dry ice; store in liquid nitrogen
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