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Hydroxyphenyl fluorescein (HPF) and Aminophenyl fluorescein (APF) are selective for the detection of highly reactive oxygen spe-cies (hROS). HPF has little reactivity towards other ROS such as: singlet oxygen (O2), superoxide (O2-•), hydrogen peroxide (H2O2), nitric oxide (NO•), and alkyl peroxide (RO2•). HPF/APF are cell permeable and can be used in combination to detect hypo-chlorite (-OCl) production in cells. Hypochlorite can be detected by loading two samples, one with APF and the other with HPF. Hypo-chlorite production is visualized by increase in fluorescence of APF loaded cells and no increase in fluorescence in HPF loaded cells.
https://www.creativebiomart.net/Hypochlorite-Detection-Kit-463166.htm
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