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Description
The HeLa 382 cell line is a subclone of the cell line HeLa. The original HeLa cell line was derived from a cervical carcinoma from a 31 year old female and the HeLa 382 cell line was transfected with pBact, expression vector under control of the human beta actin promotor, resistant to G418S. Control to HeLa 422 and HeLa 432.
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Description
The HeLa 382 cell line is a subclone of the cell line HeLa. The original HeLa cell line was derived from a cervical carcinoma from a 31 year old female and the HeLa 382 cell line was transfected with pBact, expression vector under control of the human beta actin promotor, resistant to G418S. Control to HeLa 422 and HeLa 432.
Organism: Homo sapiens, human
Source: cervix
Culture Properties: Monolayer
Morphology: continuous culture, grown as monolayer, morphology epithelial-like
Recommended Medium: EMEM and 10% h.i. FBS
Quality ControlSterility: mycoplasma negative, HOECHST and PCR
Storage and Shipping: Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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